Oct 20, 2009 · Equine Semen Extenders: Effect on Bacterial Growth and Sperm Viability. Preliminary results of a collaborative effort between researchers at Oklahoma State University and Texas A&M University were ...
The viability of sperm is an important aspect of ejaculate quality that determines competitive fertilization success in the field cricket Teleogryllus oceanicus. Using in vitro mixtures of sperm and seminal fluid from pairs of male crickets, we show that seminal fluid can affect the viability of sperm in this species.
Feb 09, 2014 · 4.1. Sperm Motility, Number and Viability The results of mean sperm motility, number and viability are presented in Figures 1-3, respectively. a) Effects of sertraline on sperm motility: There is a significant decrease in motility of sperm. Three doses of ser- traline (0.0534 mg/kg) per day have the most significant effect on decreasing the ...
decreased sperm motility and viability. LC did not alter the baseline of sperm DNA oxidation during both incubation and centrifugation. In conclusion, LC may enhance sperm motility following incubation and centrifugation, while it might not affect sperm viability and DNA oxidation. ª 2011 Blackwell Verlag GmbH Andrologia 2012, 44, 505–512 505
Sep 22, 2021 · causes irreversible damage to sperm that can affect its ability to fertilize and generate a viable. ... motility and viability of cryopreserved ram semen and decreases lipid peroxidation in.
Sep 12, 2019 · Furthermore, Locke and Peng found that aging affects drone sperm viability, which decreased to 86% in 14-day-old drones and 81% in 20-day-old drones. Likewise, Stürup et al. ( 2013 ) found that drones that survived more than 20 days post emergence exhibited as much as 50% lower sperm viability than those that survived less than 20 days.
May 01, 2020 · Moradi et al. (2013) examining the protective properties of RJ during sperm storage for up to 120 h described that its addition caused an increase in sperm viability depending on RJ concentration and length of storage. As in case of sperm morphology, the type of semen extender and storage time affect the kinetic values of sperm.
Ergothioneine improves the total motility and viability of cryopreserved ram semen and decreases lipid peroxidation in cryopreserved rooster and ram sperm [14,15]. An important advantage of ERG over other antioxidants is that it does not interfere with the important roles of ROS but acts when oxidative damage becomes excessive [ 18 ].
Aim: The goal was to compare the effects of three different sperm preparation media on sperm motility, viability, and DNA integrity of semen samples from normozoospermic men.Methods: A total of 15 normozoospermic males were included in the study. The semen analysis (SA) was performed in accordance with the WHO guidelines (2010). After SA, each sample was divided into three aliquots, …
Mar 14, 2016 · On the other hand, it is assumed that storage period in deep freezing does not affect sperm viability [24, 25], and there is argument that spermatozoa retain their fertilizing potential indefinitely when stored at −79°C in dry ice, or at −196°C in liquid nitrogen . However, there is a scarcity of studies designed in order to detect a ...
In equids, they are utilized for their ability to maintain plasma membrane integrity while protecting spermatozoa from cold shock, detrimental seminal plasma proteins, and toxic byproducts produced by the sperm cells. Available from:. One frame containing honey and pollen and a small group of young workers was included in each nuc box to tend to new drones as they emerged. The quality of sperm preparation medium affects the motility, viability, and DNA integrity of human spermatozoa. Approximately 30 sexually mature drones were collected from each colony 14 and 20 days after emergence to assess parameters such as body weight, forewing length, and sperm counts. Honey bee Apis mellifera colonies invest a substantial amount of colony resources in the production of drones during the reproductive season to enable mating with virgin queens from nearby colonies. Cobey, S. We would like to thank Gene ET Ash for his advice and assistance in drone rearing and collection. Sperm swim-up techniques and DNA fragmentation. Table I Summary of values obtained from studies that have explored the effects of age, season, and genetics on the reproductive health of honey bee drones Full size table. This indicates a method by which drones communicate the availability of a visiting queen that is still receptive to mating Koeniger , suggesting that cooperative behavior may occur among drones. Google Scholar Grassl, J. Collins, A. Burley LM. Flow cytometry evidence about sperm competition in honey bee Apis mellifera. View Article Google Scholar 7. Apidologie 35 , — These researchers compared antibiotic properties of common equine semen extenders with four different strains of bacteria, including Taylorella Posted by Kimberly Brown Oct 20, Article. Antimicrobial Lab Testing. There is, however, variation in the size of drone cells Berg ; Berg et al. A sperm cell that is alive will have a solid cellular membrane. Sperm must be viable and healthy to reach an egg. There are multiple types of these extenders available on the market, but results on their benefits and detriments are not definitive. The WHO guidelines are based on percentiles which are based on a group of men who fathered children in a year or less. Apimondia Publishing House, Bucharest, 22— World collaborative report on assisted reproductive technology, Abdelkader, F. Hoage, T. However, drones in the treatment group lived longer on average 21 days post emergence and had significantly lower sperm viability, than those in the control group, which lived only 15 days post emergence, on average Table IV. Sperm quality analysis of seminal doses 2. Industry News. Similarly, Rhodes et al. However, no significant differences were observed in terms of sperm counts, viability, and concentration, regardless of whether the drones had limited or unlimited access to pollen. Google Scholar Haarmann, T. Fisher II, A. Role of oocyte morphology on fertilization and embryo formation in assisted reproductive techniques. Iatrogenic DNA damage induced in human spermatozoa during sperm preparation: Protective significance of seminal plasma. Table II Summary of values obtained from studies that have explored the effects of temperature on the reproductive health of drones Full size table. These researchers compared antibiotic properties of common equine semen extenders with four different strains of bacteria, including Taylorella. Therefore, factors affecting drone health and reproductive competency may directly affect queen fitness and longevity, having great implications at the colony level. Google Scholar Abou-Shaara, H. What It Is: Semen pH is a measurement of how acidic or alkaline the semen is. Most importantly, they found that Climate, nutrition, and other environmental factors also affect the timing of drone sexual maturation Rhodes Materials and methods This study was conducted during the and reproductive seasons at the Janice and John G. If delayed liquefaction occurs, your doctor might want to follow up with a post-coital test PCT. View Article Google Scholar 2. Flow cytometry as an estimation tool for honey bee sperm viability. Ruttner, F. Frames in the control group were sprayed with 10 mL of acetone on both sides using a mL all-purpose sprayer.
Sperm viability is important especially to a couple that is having trouble conceiving a child. It is in a simplified definition viability is whether or not a person has sperm that is alive. While a typical male has seminal fluid that has a large amount of sperm not all of the sperm are viable or alive. There are many factors that affect whether a sperm is viable. It does not stop at the simple fact of if the sperm is alive or dead. Fertility is much more complicated than if sperm is viable or not. A test for fertility is successful if a man's sperm comes back and is at least sixty percent viable. To test if a man's sperm is viable doctors and scientists use a sample of seminal fluid. This seminal fluid is measured to see what sperm is alive and what is dead. A common way to test if the sperm is dead or alive is to use a dye in the sperm. The non-viable sperm cells, the dead sperm cells, will absorb the dye. This is because these cells will not have a solid cellular membrane. This allows the dye to leak into the cell thus dying it. A sperm cell that is alive will have a solid cellular membrane. Which will protect the cell from absorbing the dye. Image source: here. Looking past whether sperm is alive or dead viability focuses on the sperm cells that are alive. These sperm cells must be healthy. Any sperm cells that are damaged can make it hard to conceive a child. The damage, or defects, could be a number of problems. The sperm may have its DNA altered making it non-viable. If there any antibodies present that fight sperm it can make it difficult for the sperm to fertilize an egg. Other chemicals can affect sperm and make it impossible for the sperm to fertilize an egg. Sperm must be viable and healthy to reach an egg. The vagina is an acidic environment and it will kill the sperm as it travels to fertilize the egg. If the seminal fluid is not viable or it has a low amount of viable sperm it will be improbable that the sperm will reach the egg before dying. If there are factors that have decreased sperm viability it is likely the man will have trouble conceiving a child. Fertility must include factor more in depth than just sperm viability. Viability of sperm is important but the sperm must also be motile. If the sperm has low mobility it can be difficult for the sperm to reach an egg before it is killed by the hostile environment of the vagina. Another aspect that is look at is the total sperm count. If a man has a high sperm count it can help to combat if his sperm has lower viability. It is the same if the sperm count is low. Even if they have viable sperm with a low sperm count it can still be difficult for the sperm to fertilize an egg. Close menu. For iPhone. For Most Other Phones. For iPad and Most Other Tablets. For Laptop. Lab Testing. Antimicrobial Lab Testing. Military Grade Drop Testing. Is Cell Phone Radiation Harmful? Verified Reviews. News Coverage. About Us.
Google Scholar Corta, E. View Article Google Scholar 3. A high fever can also affect your sperm counts. However, the impact of age on drone reproductive quality appears to be highly variable, and it is not always negative. Shoukry et al. Reproductive behavior Climate, nutrition, and other environmental factors also affect the timing of drone sexual maturation Rhodes The results shown differ from those obtained by Malik et al. Google Scholar Lodesani, M. Contrary, field trial results of Strom [ 14 ] found no evidence of reduced fertility when was used frozen semen storage by 1—1. Thus, drones experiencing a reduction in sperm viability or sperm count from a variety of factors Rinderer et al. Ciereszko et al. Influence of sperm chromatin anomalies on assisted reproductive technology outcome. View author publications. Results: There were no significant differences, at any time intervals, in the total sperm motility between the different sperm preparation media. In a study examining commercial beekeeping operations in the United States, Mullin et al. Because drones are the source of half of the genetic material required for the production of female workers, a queen that mates with substandard drones may run out of viable sperm sooner than normal. Effects of age, season and genetics on semen and sperm production in Apis mellifera drones. Tarpy, D. Free, J. Most importantly, they found that Moreover, Amann [ 41 ] reported a high level of correlation between competitive fertility index and sperm motility, VCL, VSL parameters, with 0. Drones were reared in plastic drone frames placed in recently re-queened colonies that had not been treated with either fluvalinate or coumaphos for at least 5 years. These pesticides include the miticides fluvalinate, coumaphos and amitraz, and the agro-chemicals chlorothalonil and chlorpyrifos. For iPad and Most Other Tablets. Each frame was then allocated to an experimental group. Kimberly Brown. Google Scholar Locke, S. J Androl ; Abou-Shaara, H. A Plasma membrane integrity analysis, green or red fluorescents marks correspond to sperm recognized as live or dead, respectively. There are a variety of causes for necrozoospermia, including many of the same things that can cause low sperm count. J of Ap Res. To prepare the frames used for drone rearing, approximately 20 lbs of organic, cosmetic grade beeswax pellets Koster Keunen Inc. Locke, S. These researchers compared antibiotic properties of common equine semen extenders with four different strains of bacteria, including Taylorella Posted by Kimberly Brown Oct 20, Article. Academic Press, Inc. The sperm may have its DNA altered making it non-viable. A common way to test if the sperm is dead or alive is to use a dye in the sperm. The frames were previously coated with melted beeswax following the procedures outlined below. Int J Androl. Bee World 39 , 3— J Ins Physiol. Fluvalinate, coumaphos, amitraz, thymol, fenpyroximate, and oxalic acid were diluted individually in acetone at concentrations below the LD 10 rate for worker bees. Fisher II, A. Search SpringerLink Search. Apidologie 50, — They may or may not have materials to help inspire you for the collection, so you might want to bring a magazine or your smartphone.
Today, it is theoretically assumed that frozen storage of semen doses in liquid nitrogen guarantees sperm functionality indefinitely. Sperm viability and full sperm motility were analyzed by CASA system, and acrosome integrity was assessed with Coomassie blue staining. However, future field trials will be necessary in order to validate that both fertility and embryo viability are maintained for the times analyzed. Cryopreservation in Eukaryotes. Cryopreservation protocols for the bull used in the animal production industry began in the s [ 1 ]. Since then, both the packaging type and cryopreservation system were changing on the time. Primary considerations in the selection of a system for packaging semen were fertility, insemination preference, ease of handling, ease of identification, freedom from contamination, economics of storage and efficiency of ejaculates [ 2 ]. Sperm are commonly packaged in one of three ways: a glass ampoules, normally containing 0. Regarding to time storage factor, studies of sperm motility have indicated a descent in sperm viability after storage [ 4 , 5 ]. However, an inverse relationship between preservation of sperm viability and storage temperature was shown [ 7 ]. Furthermore, since the s, there have been mentions that deterioration continues even when sperm are stored in liquid nitrogen; suspecting that aging of spermatozoa may occur if semen is stored for long periods of time, and this may be associated with embryo mortality and delayed return [ 9 , 15 ]. Field trials using frozen semen packaged in ampoules, pellets and straws have indicated that the influence of packaging methods in fertility has been inconsistent [ 2 ]. Therefore, according to these authors, if the semen fertility stored in LN is reduced with time, regardless of packaging technique, some factors other than storage are responsible. This is a highly practical method in breeding programs for domestic animals and is used to maintain the establishment and genetic diversity of gene banks [ 20 , 21 ]. However, there is a scarcity of studies designed in order to detect a decrease in reproductive performance of cryopreserved semen as a function of storage time. Then the question arises, how long cells can be stored in liquid nitrogen without suffering damage? There is the possibility of slow accumulation of direct damage from ionizing radiation, but this becomes significant only after centuries of storage [ 29 ]. However, other evidence suggests that this could be due to inadequate maintenance of temperatures [ 26 ]. Leibo et al. In this study, a total of 75 commercial doses from bulls Friesian breed were used. The cryopreserved germplasm were defined and divided into five groups according to the storage time. For each group, 15 seminal doses from five different donors three each were considered. Different freezing packaging system used in this study. Permeabilized spermatozoa dried onto slides were then covered with a droplet of staining solution 0. On the other hand, comparative analysis of total sperm motility did not show significant differences between times storage analyzed. The results shown differ from those obtained by Malik et al. This could also be due to the evident differences associated with the use of analysis of subjective sperm motility analysis versus our use of CASA system. Effectively, although Mazur [ 29 ] proposed that several centuries are required, of liquid nitrogen storage, for that ionizing cosmic radiation alters or damages the DNA of the cell. Fourteen five years ago, Salisbury and Hart [ 16 ] proposed that bovine frozen sperm have a low fertility level and promote increased embryonic mortality after 1. More recently, Haugan et al. Contrary, field trial results of Strom [ 14 ] found no evidence of reduced fertility when was used frozen semen storage by 1—1. Unfortunately, there are no field trials in that and both pregnancy and calving rates have been analyzed; to rule out or confirm effects of prolonged storage on embryo mortality, this would be the only one way to resolve the question. A Plasma membrane integrity analysis, green or red fluorescents marks correspond to sperm recognized as live or dead, respectively. B Sperm motility analysis, the tracking in red, green, blue and yellow, correspond to sperm sorted according velocity: rapid, medium, slow and static, respectively. Considering that the plasma and acrosomal membrane integrity are two irreversible parameters of sperm quality, and that the motility is commonly believed to be one of the most important characteristics associated with the fertilizing ability of semen [ 38 ]. Our freezability data, analyzed as a whole, suggest that fertilizing potential of the seminal dose is commercially analyzed, independent of storage time, and it is high. In this respect, Budworth et al. Moreover, Amann [ 41 ] reported a high level of correlation between competitive fertility index and sperm motility, VCL, VSL parameters, with 0. Complementary analysis, including other aspects as to mitochondrial metabolism, reactive oxygen species ROS levels, DNA fragmentation and chromatin integrity, could shed light on possible and potential changes induced for prolonged storage. Special thanks to all professional and technical personnel of the Center of Artificial Insemination, that from the s set up and development the bovine semen cryopreservation in Chile and whose seminal doses stored for long term has made this study possible. Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution 3. 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